《植物生理学报》 2010, 46(5): 441-447

一个麻疯树胞质II 类小热激蛋白基因JcHSP17.5 的克隆和异源表达

朱勋路, 朱习红, 辜小苹, 徐莺* , 陈放

四川大学生命科学学院, 成都610065

通信作者：徐莺；E-mail: yinghsu@263.net；Tel: 028-85417281

摘　要：

从麻疯树胚乳cDNA文库中得到分子量大小约为17.5 kDa的细胞质II类小热激蛋白基因, 命名为JcHSP17.5, GenBank 登录号为GU320741。通过构建外源表达载体, 将JcHSP17.5 基因分别在大肠杆菌以及酿酒酵母中过量表达来研究基因的 耐热和耐渗透压胁迫能力, 发现两种重组菌在逆境下都较对照组生活力有显著提高。推测JcHSP17.5在麻疯树生长过程中 可能与麻疯树耐热、耐旱以及耐离子胁迫相关。

关键词：麻疯树; 小热激蛋白; 基因表达; 耐热; 耐渗透压

收稿：2010-01-15   修定：2010-04-29

资助：“ 十一五” 科技支撑项目(2006BAD07A04)和国家自然科技资源平台项目(2005DKA21403)。

Cloning and Heterologous Expression of a Cytosolic Class II Small Heat Shock Protein Gene (JcHSP17.5) from Jatropha curcas L.

ZHU Xun-Lu, ZHU Xi-Hong, GU Xiao-Ping, XU Ying*, CHEN Fang

College of Life Sciences, Sichuan University, Chengdu 610065, China

Corresponding author: XU Ying; E-mail: yinghsu@263.net; Tel: 028-85417281

Abstract:

JcHSP17.5, an approximately 17.5 kDa cytosolic class II small heat shock protein (HSP) gene was cloned from a Jatropha curcas endosperm cDNA library (GenBank accession number is GU320741). To verify its possible function, the thermally and osmotically tolerant characteristics were tested by overexpressing the recombinant JcHSP17.5 gene in Escherichia coli and Saccharomyces cerevisiae, respectively. The results showed that the recombinant cells exhibited significantly improved viability in both thermal and osmotic stress condition compared with the control. It suggested that the JcHSP17.5 represented an example of a HSP which was capable of protecting cells against environmental extreme.

Key words: Jatropha curcas; small heat shock protein; gene expression; thermotolerance; osmotolerance